Background: Theoretically, insecure attachment and negative parental behaviors are risk factors for childhood anxiety. However, few empirical studies have examined their relative contribution including differences between mothers and fathers. To date, only one study has examined a mediational model including these factors, albeit in a nonclinical sample. Method: This study ameliorates this limitation by investigating direct and indirect relations between maternal and paternal attachment and behaviors, and clinical anxiety in children (mean age 9.6 years). The study recruited 54 families. Anxiety symptoms were measured by the Spielberger State-trait Inventory for Children, attachment relationships by the Security Scale, and parental behaviors using the Rearing Behavior Questionnaire. Results: Neither insecure attachment relationship with mother nor maternal negative behavior was a significant predictor of anxiety in children. However, insecure attachment to father accounted for a significant proportion of variance in anxiety, and also fully mediated the relationship between paternal rejection and anxiety. Conclusions: Our findings highlight the importance of assessing the relative contribution of risk factors and the importance of including fathers when investigating the development and maintenance of childhood anxiety.